产品名称
ANKT Rabbit Polyclonal Antibody
别名
NUSAP1; ANKT; BM-037; Nucleolar and spindle-associated protein 1; NuSAP
蛋白名称
Nucleolar and spindle-associated protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51203
Human Swissprot No.
Q9BXS6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BXS6/entry
Mouse Swissprot No.
Q9ERH4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ERH4
免疫原
The antiserum was produced against synthesized peptide derived from human NUSAP1. AA range:392-441
特异性
ANKT Polyclonal Antibody detects endogenous levels of ANKT protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009],
组织表达
Bone marrow,Cervix,Epithelium,Fetal liver,Liver,Lung,Lymph,
细胞定位
Cytoplasm . Nucleus, nucleolus . Cytoplasm, cytoskeleton, spindle . Chromosome . Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis. .
功能
domain:The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.,function:Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.,similarity:Belongs to the NUSAP family.,subcellular location:Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.,subunit:Interacts with DNA and microtubules. Microtubule bundling is inhibited by IPO7, KPNA2 and KPNB1 while association with DNA is also inhibited by IPO7 and KPNA2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
