BD-PT0216-AMPKα1/2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AMPKα1/2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0216

产品名称

AMPKα1/2 Rabbit Polyclonal Antibody

别名

PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

类别

常规抗体

基因名称

AAPK1/AAPK2

蛋白名称

5'-AMP-activated protein kinase catalytic subunit alpha-1/2

推荐应用

反应种属

Human,Mouse,Rat,Monkey,Bovine,Fish

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5562/5563

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5562

Human Swissprot No.

Q13131/P54646

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13131/entry

Mouse Gene ID

105787/108079

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=105787

Rat Gene ID

65248/78975

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=65248

Rat Swissprot No.

P54645/Q09137

Rat Swissprot Link

http://www.uniprot.org/uniprot/P54645

免疫原

The antiserum was produced against synthesized peptide derived from human AMPK alpha. AA range:140-189

特异性

AMPKα1/2 Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.

稀释度

WB 1:500-2000;IHC-p 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

预测分子量

63kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

组织表达

Brain,Intestine,Liver,Mammary gland,Platelet,Testis

细胞定位

Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .

信号通路

Insulin Receptor; mTOR; AMPK

功能

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, treated with Adriamycin 0.5ng/ml 24h, using AMPK alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using AMPKα1/2 Polyclonal Antibody diluted at 1：500

Western Blot analysis of COS7 cells using AMPKα1/2 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.