产品名称
AMID Rabbit Polyclonal Antibody
别名
AIFM2; AMID; PRG3; Apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein
蛋白名称
Apoptosis-inducing factor 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84883
Human Swissprot No.
Q9BRQ8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BRQ8/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=71361
Mouse Swissprot No.
Q8BUE4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BUE4
免疫原
The antiserum was produced against synthesized peptide derived from human AIFM2. AA range:141-190
特异性
AMID Polyclonal Antibody detects endogenous levels of AMID protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010],
组织表达
Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells.
细胞定位
Lipid droplet . Cell membrane ; Lipid-anchor . Cytoplasm . Mitochondrion membrane . Nucleus .
功能
cofactor:FAD. Binds 6-hydroxy-FAD non-covalently.,function:Oxidoreductase, which may play a role in mediating a TP53/p53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest.,induction:Expression detected at 4 hours after induction by TP53/p53. Down-regulated in a wide range of human tumors.,similarity:Belongs to the FAD-dependent oxidoreductase family.,tissue specificity:Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following TP53/p53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
