产品名称
AKAP 95 Rabbit Polyclonal Antibody
别名
AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
蛋白名称
A-kinase anchor protein 8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10270
Human Swissprot No.
O43823
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43823/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56399
Mouse Swissprot No.
Q9DBR0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9DBR0
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116633
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63014
免疫原
The antiserum was produced against synthesized peptide derived from human AKAP8. AA range:331-380
特异性
AKAP 95 Polyclonal Antibody detects endogenous levels of AKAP 95 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011],
组织表达
Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.
细胞定位
Nucleus . Nucleus matrix . Nucleus, nucleolus . Cytoplasm . Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Exhibits partial localization to the nucleolus in interphase, where it colocalizes with UBTF/UBF, suggesting localization to the fibrillary center and/or to the dense fibrillary component. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase. .
功能
function:Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).,similarity:Belongs to the AKAP95 family.,subcellular location:Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.,subunit:Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis.,tissue specificity:Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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