产品名称
AKAP 10 Rabbit Polyclonal Antibody
别名
AKAP10; A-kinase anchor protein 10; mitochondrial; AKAP-10; Dual specificity A kinase-anchoring protein 2; D-AKAP-2; Protein kinase A-anchoring protein 10; PRKA10
蛋白名称
A-kinase anchor protein 10 mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11216
Human Swissprot No.
O43572
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43572/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56697
Mouse Swissprot No.
O88845
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O88845
免疫原
The antiserum was produced against synthesized peptide derived from human AKAP10. AA range:10-59
特异性
AKAP 10 Polyclonal Antibody detects endogenous levels of AKAP 10 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012],
细胞定位
Mitochondrion . Membrane . Cytoplasm . Predominantly mitochondrial but also membrane associated and cytoplasmic.
功能
domain:RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.,function:Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase.,similarity:Contains 2 RGS domains.,subcellular location:Predominantly mitochondrial but also membrane associated and cytoplasmic.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
