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ADCK4 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT0122
产品名称
ADCK4 Rabbit Polyclonal Antibody
别名
ADCK4; Uncharacterized aarF domain-containing protein kinase 4
类别
常规抗体
基因名称
ADCK4
蛋白名称
Uncharacterized aarF domain-containing protein kinase 4
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
79934
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=79934
Human Swissprot No.
Q96D53
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96D53/entry
Mouse Swissprot No.
Q566J8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q566J8
Rat Gene ID
308453
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=308453
Rat Swissprot No.
Q6AY19
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q6AY19
免疫原
The antiserum was produced against synthesized peptide derived from human ADCK4. AA range:31-80
特异性
ADCK4 Polyclonal Antibody detects endogenous levels of ADCK4 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
预测分子量
65kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
组织表达
Widely expressed, including renal podocytes.
细胞定位
Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm, cytosol . Cell membrane .
功能
function:The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).,similarity:Belongs to the protein kinase superfamily. ADCK protein kinase family.,similarity:Contains 1 protein kinase domain.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using ADCK4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using ADCK4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using ADCK4 antibody.

Western Blot analysis of various cells using ADCK4 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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