BD-PT0118

ADAR1 Rabbit Polyclonal Antibody

ADAR; ADAR1; DSRAD; G1P1; IFI4; Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP

常规抗体

ADAR

Double-stranded RNA-specific adenosine deaminase

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

103

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=103

P55265

http://www.uniprot.org/uniprotkb/P55265/entry

56417

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56417

Q99MU3

http://www.uniprot.org/uniprot/Q99MU3

81635

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81635

P55266

http://www.uniprot.org/uniprot/P55266

The antiserum was produced against synthesized peptide derived from human ADAR1. AA range:1172-1221

ADAR1 Polyclonal Antibody detects endogenous levels of ADAR1 protein.

WB 1:500-2000

135kD

-20°C/1 year

Polyclonal, Rabbit,IgG

adenosine deaminase, RNA specific(ADAR) Homo sapiens This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010],

Ubiquitously expressed, highest levels were found in brain and lung (PubMed:7972084). Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.

[Isoform 1]: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus (PubMed:7565688, PubMed:24753571). Nuclear import is mediated by TNPO1 (PubMed:24753571). .; [Isoform 5]: Cytoplasm . Nucleus . Nucleus, nucleolus . Predominantly nuclear but can shuttle between nucleus and cytoplasm. TNPO1 can mediate its nuclear import whereas XPO5 can mediate its nuclear export. .

Cytosolic DNA-sensing pathway;

caution:The N-terminus of isoform 4 has been derived from EST and genomic sequences.,disease:Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.,function:Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.,induction:Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed.,PTM:Sumoylation reduces RNA-editing activity.,similarity:Contains 1 A to I editase domain.,similarity:Contains 2 DRADA repeats.,similarity:Contains 3 DRBM (double-stranded RNA-binding) domains.,subcellular location:Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.,subunit:Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90.,tissue specificity:Ubiquitously expressed, highest levels were found in brain and lung.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.