BD-PT0088-ACOT4 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ACOT4 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT0088

产品名称

ACOT4 Rabbit Polyclonal Antibody

别名

ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

类别

常规抗体

基因名称

ACOT4

蛋白名称

Acyl-coenzyme A thioesterase 4

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

122970

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=122970

Human Swissprot No.

Q8N9L9

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q8N9L9/entry

Mouse Swissprot No.

Q8BWN8

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8BWN8

免疫原

The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410

特异性

ACOT4 Polyclonal Antibody detects endogenous levels of ACOT4 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,

组织表达

Strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle.

细胞定位

Peroxisome .

信号通路

Biosynthesis of unsaturated fatty acids;

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from COLO205 cells using ACOT4 antibody.

Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.