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ACOT1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT0085
产品名称
ACOT1 Rabbit Polyclonal Antibody
别名
ACOT1; CTE1; Acyl-coenzyme A thioesterase 1; Acyl-CoA thioesterase 1; CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase
类别
常规抗体
基因名称
ACOT1
蛋白名称
Acyl-coenzyme A thioesterase 1
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
641371
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=641371
Human Swissprot No.
Q86TX2
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q86TX2/entry
Mouse Swissprot No.
O55137
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O55137
免疫原
The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140
特异性
ACOT1 Polyclonal Antibody detects endogenous levels of ACOT1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
46kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
组织表达
Neuroblastoma,
细胞定位
Cytoplasm, cytosol .
信号通路
Biosynthesis of unsaturated fatty acids;
功能
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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