产品名称
AAK1 Rabbit Polyclonal Antibody
别名
AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
蛋白名称
AP2-associated protein kinase 1
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22848
Human Swissprot No.
Q2M2I8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q2M2I8/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=269774
Mouse Swissprot No.
Q3UHJ0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q3UHJ0
Rat Swissprot Link
http://www.uniprot.org/uniprot/P0C1X8
免疫原
The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320
特异性
AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],
组织表达
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
细胞定位
Cell membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Stimulated by clathrin.,function:Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Active when found in clathrin-coated pits at the plasma membrane.,subunit:Interacts with alpha-adaptin and AP-2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
