产品名称
5-LO Rabbit Polyclonal Antibody
别名
ALOX5; LOG5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase
蛋白名称
Arachidonate 5-lipoxygenase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=240
Human Swissprot No.
P09917
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09917/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11689
Mouse Swissprot No.
P48999
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P48999
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25290
Rat Swissprot Link
http://www.uniprot.org/uniprot/P12527
免疫原
The antiserum was produced against synthesized peptide derived from human Arachidonate 5 Lipoxygenase. AA range:246-295
特异性
5-LO Polyclonal Antibody detects endogenous levels of 5-LO protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
细胞定位
Cytoplasm . Nucleus matrix . Nucleus membrane ; Peripheral membrane protein . Cytoplasm, perinuclear region . Cytoplasm, cytosol . Nucleus envelope . Nucleus intermembrane space . Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640). .
信号通路
Arachidonic acid metabolism;
功能
catalytic activity:Arachidonate + O(2) = leukotriene A(4) + H(2)O.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 2 calcium ions per subunit.,function:Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.,pathway:Lipid metabolism; leukotriene A4 biosynthesis.,PTM:Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.,similarity:Belongs to the lipoxygenase family.,similarity:Contains 1 lipoxygenase domain.,similarity:Contains 1 PLAT domain.,subcellular location:Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.,subunit:Interacts with ALOX5AP and LTC4S.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
