产品名称
4E-BP1 Rabbit Polyclonal Antibody
别名
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
蛋白名称
Eukaryotic translation initiation factor 4E-binding protein 1
反应种属
Human,Mouse,Rat,Sheep
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1978
Human Swissprot No.
Q13541
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13541/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13685
Mouse Swissprot No.
Q60876
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q60876
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116636
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q62622
免疫原
The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:13-62
特异性
4E-BP1 Polyclonal Antibody detects endogenous levels of 4E-BP1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],
组织表达
Colon,Epithelium,Lung,Placenta,Platelet,
细胞定位
nucleoplasm,cytoplasm,cytosol,protein complex,
信号通路
Regulates Angiogenesis; Insulin Receptor; mTOR; ErbB/HER; PI3K/Akt; AMPK
功能
function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
