产品名称
14-3-3 θ Rabbit Polyclonal Antibody
别名
YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
蛋白名称
14-3-3 protein theta
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10971
Human Swissprot No.
P27348
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P27348/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22630
Mouse Swissprot No.
P68254
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P68254
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25577
Rat Swissprot Link
http://www.uniprot.org/uniprot/P68255
免疫原
The antiserum was produced against synthesized peptide derived from human 14-3-3 thet. AA range:41-90
特异性
14-3-3 θ Polyclonal Antibody detects endogenous levels of 14-3-3 θ protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008],
组织表达
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
细胞定位
Cytoplasm. In neurons, axonally transported to the nerve terminals.
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Neurotrophin;Pathogenic Escherichia coli infection;
功能
function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:In neurons, axonally transported to the nerve terminals.,subunit:Homodimer. Interacts with PCTK1 (By similarity). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.,tissue specificity:Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
