BD-PT0008

14-3-3 ζ/δ Rabbit Polyclonal Antibody

YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

常规抗体

YWHAZ

14-3-3 protein zeta/delta

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

7534

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7534

P63104

http://www.uniprot.org/uniprotkb/P63104/entry

22631

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22631

P63101

http://www.uniprot.org/uniprot/P63101

25578

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25578

P63102

http://www.uniprot.org/uniprot/P63102

The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta. AA range:196-245

14-3-3 ζ/δ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ/δ protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

28kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

B-cell lymphoma,Bone marrow

Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Neurotrophin;Pathogenic Escherichia coli infection;

caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to stage I to stage IV melanosomes.,subunit:Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with PCTK1 and BSPRY (By similarity). Interacts with WEE1 (C-terminal) (By similarity). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional acivity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with AANAT ('Thr-31' phosphorylated form); the interaction modulates AANAT enzymatic activity through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT ('Ser-205' phosphorylated form), can bind the other YWHAZ monomer with similar effect. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.