产品名称
DAPK1 (Phospho-Ser308) Rabbit Polyclonal Antibody
别名
Death-associated protein kinase 1 (DAP kinase 1;EC 2.7.11.1)
蛋白名称
DAPK1 (Phospho-Ser308)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Swissprot No.
P53355
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P53355/entry
Rat Swissprot Link
Human:S308, Mouse:S308
免疫原
Synthesized peptide derived from human DAPK1 (Phospho-Ser308)
特异性
This antibody detects endogenous phospho levels of DAPK1 (Phospho-Ser308) at Human:S308, Mouse:S308
稀释度
IHC-p 1:50-200, WB 1:500-2000. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
组织表达
Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
细胞定位
[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
