产品名称
MLK3 (phospho Ser674) Rabbit Polyclonal Antibody
别名
MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
蛋白名称
Mitogen-activated protein kinase kinase kinase 11
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4296
Human Swissprot No.
Q16584
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16584/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26403
Mouse Swissprot No.
Q80XI6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80XI6
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=309168
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q66HA1
免疫原
Synthesized phospho-peptide around the phosphorylation site of human MLK3 (phospho Ser674)
特异性
Phospho-MLK3 (S674) Polyclonal Antibody detects endogenous levels of MLK3 protein only when phosphorylated at S674.
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],
组织表达
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subcellular location:Location is cell cycle dependent.,subunit:Homodimer; undergoes dimerization during activation.,tissue specificity:Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
