BD-PP1197

ERK 1/2 (phospho Thr202/Y204) Rabbit Polyclonal Antibody

MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p

常规抗体

MAPK1/MAPK3

Mitogen-activated protein kinase 3

WB

Human,Mouse,Rat,Fish

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

5595/5594

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5595

P27361/P28482

http://www.uniprot.org/uniprotkb/P27361/entry

26417/26413

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26417

50689/116590

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=50689

P21708/P63086

http://www.uniprot.org/uniprot/P21708

Synthesized phospho-peptide around the phosphorylation site of human ERK 1/2 (phospho Thr202/Y204)

Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202 or Y204.

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.

43kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

Epithelium,Eye,Hepatoma,Human cervix,Lymph,

Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .

MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;Chemokine;Oocyte meiosis;mTOR;Vascular smooth muscle contraction;Dorso-ventral axis formation;TGF-beta;Axon guidance;VEGF;Focal adhesion;Adherens_Junction;Gap junction;Toll_Like;NOD-like receptor;Natural killer cell

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF.,function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,PTM:Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.