BD-PP1193

EDG-1 (phospho Thr236) Rabbit Polyclonal Antibody

S1PR1; CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD antigen CD363

常规抗体

S1PR1

Sphingosine 1-phosphate receptor 1

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1901

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1901

P21453

http://www.uniprot.org/uniprotkb/P21453/entry

13609

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13609

O08530

http://www.uniprot.org/uniprot/O08530

29733

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29733

P48303

http://www.uniprot.org/uniprot/P48303

The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255

Phospho-EDG-1 (T236) Polyclonal Antibody detects endogenous levels of EDG-1 protein only when phosphorylated at T236.

WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

42kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.

Neuroactive ligand-receptor interaction;

function:Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.,induction:By the tumor promoter phorbol 12-myristate 13-acetate (PME) in the presence of cycloheximide.,PTM:S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.