产品名称
PLB (phospho Ser16/T17) Rabbit Polyclonal Antibody
别名
PLN; PLB; Cardiac phospholamban; PLB
蛋白名称
Cardiac phospholamban
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5350
Human Swissprot No.
P26678
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P26678/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18821
Mouse Swissprot No.
P61014
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P61014
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64672
Rat Swissprot Link
http://www.uniprot.org/uniprot/P61016
免疫原
The antiserum was produced against synthesized peptide derived from human PLB around the phosphorylation site of Ser16 and Thr17. AA range:1-50
特异性
Phospho-PLB (S16/T17) Polyclonal Antibody detects endogenous levels of PLB protein only when phosphorylated at S16/T17.
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016],
组织表达
Heart muscle (at protein level).
细胞定位
Endoplasmic reticulum membrane ; Single-pass membrane protein . Sarcoplasmic reticulum membrane ; Single-pass membrane protein . Mitochondrion membrane ; Single-pass membrane protein . Membrane ; Single-pass membrane protein . Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). .
信号通路
Calcium;Dilated cardiomyopathy;
功能
disease:Defects in PLN are the cause of cardiomyopathy dilated type 1P (CMD1P) [MIM:609909]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.,PTM:Phosphorylated in response to beta-adrenergic stimulation.,similarity:Belongs to the phospholamban family.,subunit:Homopentamer.,tissue specificity:Heart.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
