产品名称
Vinculin (phospho Tyr821) Rabbit Polyclonal Antibody
别名
VCL; Vinculin; Metavinculin
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7414
Human Swissprot No.
P18206
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P18206/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22330
Mouse Swissprot No.
Q64727
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64727
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=305679
Rat Swissprot Link
http://www.uniprot.org/uniprot/P85972
免疫原
The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835
特异性
Phospho-Vinculin (Y821) Polyclonal Antibody detects endogenous levels of Vinculin protein only when phosphorylated at Y821.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],
组织表达
Metavinculin is muscle-specific.
细胞定位
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .
信号通路
Focal adhesion;Adherens_Junction;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;
功能
disease:Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.,online information:Vinculin entry,PTM:Aceylated; mainly by myristic acid but also small amount of palmitic acid.,PTM:Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.,similarity:Belongs to the vinculin/alpha-catenin family.,subcellular location:Cytoplasmic face of adhesion plaques.,subunit:Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1. Interacts with SYNM.,tissue specificity:Metavinculin is muscle-specific.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
