产品名称
NMDAε1/2 (phospho Tyr1246/1252) Rabbit Polyclonal Antibody
别名
GRIN2A; NMDAR2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A; GRIN2B; NMDAR2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; N
蛋白名称
Glutamate [NMDA] receptor subunit epsilon-1/2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2903
Human Swissprot No.
Q12879/Q13224
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q12879/entry
Mouse Gene ID
14811/14812
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14811
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24409
Rat Swissprot No.
Q00959/Q00960
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q00959
免疫原
The antiserum was produced against synthesized peptide derived from human NMDAR2A/B around the phosphorylation site of Tyr1246/1252. AA range:1216-1265
特异性
Phospho-NMDAε1/2 (Y1246/1252) Polyclonal Antibody detects endogenous levels of NMDAε1/2 protein only when phosphorylated at Y1246/1252.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
组织表达
Brain,Cerebellum,Epithelium,Hippocampus,
细胞定位
Cell projection, dendritic spine . Cell membrane ; Multi-pass membrane protein . Cell junction, synapse . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane . Expression at the dendrite cell membrane and at synapses is regulated by SORCS2 and the retromer complex. .
信号通路
Calcium;Neuroactive ligand-receptor interaction;Long-term potentiation;Alzheimer's disease;Amyotrophic lateral sclerosis (ALS);Systemic lupus erythematosus;
功能
function:NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subunit:Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4. Interacts with HIP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
