产品名称
mTOR (phospho Ser2481) Rabbit Polyclonal Antibody
别名
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar
蛋白名称
Serine/threonine-protein kinase mTOR
反应种属
Human,Mouse,Rat,Bovine
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2475
Human Swissprot No.
P42345
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P42345/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56717
Mouse Swissprot No.
Q9JLN9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JLN9
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56718
Rat Swissprot Link
http://www.uniprot.org/uniprot/P42346
免疫原
The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2481. AA range:2447-2496
特异性
Phospho-mTOR (S2481) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at S2481.
稀释度
IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. ,WB 1:500-2000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],
组织表达
Expressed in numerous tissues, with highest levels in testis.
细胞定位
Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .
信号通路
Regulates Angiogenesis; Insulin Receptor; ErbB/HER; mTOR; B Cell Receptor; PI3K/Akt; AMPK
功能
function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
