产品名称
TIRAP (phospho Tyr86) Rabbit Polyclonal Antibody
别名
TIRAP; MAL; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein
蛋白名称
Toll/interleukin-1 receptor domain-containing adapter protein
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114609
Human Swissprot No.
P58753
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P58753/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117149
Mouse Swissprot No.
Q99JY1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99JY1
免疫原
The antiserum was produced against synthesized peptide derived from human TIRAP around the phosphorylation site of Tyr86. AA range:52-101
特异性
Phospho-TIRAP (Y86) Polyclonal Antibody detects endogenous levels of TIRAP protein only when phosphorylated at Y86.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:5000. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
组织表达
Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
细胞定位
Cytoplasm . Cell membrane . Membrane . Colocalizes with DAB2IP at the plasma membrane.
功能
function:Adapter involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, resulting in cytokine secretion and the inflammatory response.,polymorphism:Genetic variation in TIRAP can influence susceptibility or resistance to invasive pneumococcal disease, bacteremia, malaria and tuberculosi.,similarity:Contains 1 TIR domain.,subunit:Homodimer. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9.,tissue specificity:Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
