产品名称
IFN-α/βRα (phospho Tyr466) Rabbit Polyclonal Antibody
别名
IFNAR1; IFNAR; Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1
蛋白名称
Interferon alpha/beta receptor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3454
Human Swissprot No.
P17181
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P17181/entry
Mouse Swissprot No.
P33896
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P33896
免疫原
The antiserum was produced against synthesized peptide derived from human Interferon-alpha/beta Receptor alpha around the phosphorylation site of Tyr466. AA range:436-485
特异性
Phospho-IFN-α/βRα (Y466) Polyclonal Antibody detects endogenous levels of IFN-α/βRα protein only when phosphorylated at Y466.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008],
组织表达
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
细胞定位
[Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .
信号通路
Cytokine-cytokine receptor interaction;Toll_Like;Jak_STAT;Natural killer cell mediated cytotoxicity;
功能
function:Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.,PTM:Phosphorylated on tyrosine residues by TYK2 tyrosine kinase.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the type II cytokine receptor family.,similarity:Contains 3 fibronectin type-III domains.,tissue specificity:IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R, isoform 1 is not expressed in U266R.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
