产品名称
PTPRH Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5794
Human Swissprot No.
Q9HD43
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9HD43/entry
免疫原
Synthesized peptide derived from human PTPRH AA range: 1031-1081
特异性
This antibody detects endogenous levels of PTPRH at Human
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009],
组织表达
Expressed at high levels in the brain, spleen and liver and at lower levels in the heart and stomach. Expressed in pancreatic and colorectal cancer cells, but not in normal pancreas or colon. Expression in hepatocellular carcinoma is related to the differentiation status of the tumor and expression is inversely related to tumor aggressiveness.
细胞定位
Cell projection, microvillus membrane ; Single-pass type I membrane protein . Apical cell membrane ; Single-pass type I membrane protein . Cytoplasm . Colocalizes with CEACAM20 at the apical brush border of intestinal cells. .
功能
catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,domain:The cytoplasmic domain mediates the interaction with LCK.,domain:The extracellular domain mediates homodimerization. One or more cysteines in the extracelllular domain is essential for the formation of dimers probably by forming a disulfide bond.,enzyme regulation:Regulated by reversible dimerization. Dimerization reduces its catalytic activity.,function:May contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion-associated substrates and thus negatively regulating integrin-promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP-70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of p130cas, focal adhesion kinase and c-Src. Reduces migratory activity of Jurkat cells.,induction:Induced at the early stage of hepatocellular carcinoma and is suppressed at later stages.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,similarity:Contains 8 fibronectin type-III domains.,subunit:Homodimer; disulfide-linked (Probable). Interacts with LCK.,tissue specificity:Expressed at high levels in the brain, spleen and liver and at a lower levels in the heart and stomach. Expressed in pancreatic and colorectal cancer cells, but not in normal pancreas or colon. Expression in hepatocellular carcinoma is related to the differentiation status of the tumor and expression is inversely related to tumor aggressiveness.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
