BD-PP0936-IL-8Rβ (phospho Ser347) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IL-8Rβ (phospho Ser347) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0936

产品名称

IL-8Rβ (phospho Ser347) Rabbit Polyclonal Antibody

别名

CXCR2; IL8RB; C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; CDw128b; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2; CD antigen CD182

类别

常规抗体

基因名称

CXCR2

蛋白名称

C-X-C chemokine receptor type 2

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3579

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3579

Human Swissprot No.

P25025

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P25025/entry

Mouse Gene ID

12765

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12765

Mouse Swissprot No.

P35343

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P35343

免疫原

The antiserum was produced against synthesized peptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of Ser347. AA range:311-360

特异性

Phospho-IL-8Rβ (S347) Polyclonal Antibody detects endogenous levels of IL-8Rβ protein only when phosphorylated at S347.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

C-X-C motif chemokine receptor 2(CXCR2) Homo sapiens The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as

组织表达

Brain,Placenta,

细胞定位

Cell membrane; Multi-pass membrane protein.

信号通路

Cytokine-cytokine receptor interaction;Chemokine;Endocytosis;Epithelial cell signaling in Helicobacter pylori infection;

功能

function:Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.,online information:CXC chemokine receptors entry,PTM:Phosphorylated upon ligand binding; which is required for desensitization.,similarity:Belongs to the G-protein coupled receptor 1 family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using IL-8R beta/CDw128 beta （Phospho-Ser347） Antibody

Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta （Phospho-Ser347） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta （Phospho-Ser347） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30‘, using IL-8R beta/CDw128 beta （Phospho-Ser347） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of 3T3 cells using Phospho-IL-8Rβ （S347） Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.