BD-PP0935

Hrs (phospho Tyr334) Rabbit Polyclonal Antibody

HGS; HRS; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110

常规抗体

HGS

Hepatocyte growth factor-regulated tyrosine kinase substrate

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

9146

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9146

O14964

http://www.uniprot.org/uniprotkb/O14964/entry

15239

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15239

Q99LI8

http://www.uniprot.org/uniprot/Q99LI8

56084

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56084

Q9JJ50

http://www.uniprot.org/uniprot/Q9JJ50

The antiserum was produced against synthesized peptide derived from human HRS around the phosphorylation site of Tyr334. AA range:301-350

Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

86kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010],

Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. .

Endocytosis;

domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex.,PTM:Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 UIM (ubiquitin-interacting motif) repeat.,similarity:Contains 1 VHS domain.,subunit:Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2. Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct. Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3. Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium. Interacts with SNX1; the interaction is direct. Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR.,tissue specificity:Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.