产品名称
Stat5a/b (phospho Ser726/731) Rabbit Polyclonal Antibody
别名
STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B
蛋白名称
Signal transducer and activator of transcription 5A/B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6776
Human Swissprot No.
P42229/P51692
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P42229/entry
Mouse Gene ID
20850/20851
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20850
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24918
Rat Swissprot No.
Q62771/P52632
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q62771
免疫原
The antiserum was produced against synthesized peptide derived from human STAT5A/B around the phosphorylation site of Ser725/730. AA range:696-745
特异性
Phospho-Stat5a/b (S726/731) Polyclonal Antibody detects endogenous levels of Stat5a/b protein only when phosphorylated at S726/731.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been
组织表达
Brain,Cervix,Epithelium,Lung,Placenta,Synovial memb
细胞定位
Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation.
信号通路
ErbB_HER;Jak_STAT;Pathways in cancer;Chronic myeloid leukemia;Acute myeloid leukemia;
功能
function:Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.,online information:STAT5 entry,PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
