BD-PP0925-Histone H1 (phospho Thr17) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Histone H1 (phospho Thr17) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0925

产品名称

Histone H1 (phospho Thr17) Rabbit Polyclonal Antibody

别名

HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

类别

常规抗体

基因名称

HIST1H1B

蛋白名称

Histone H1.5

推荐应用

反应种属

Human,Mouse,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3009/3007/3008

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3009

Human Swissprot No.

P16401/P16402/P10412

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P16401/entry

Mouse Gene ID

56702/14957/50709

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56702

免疫原

The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50

特异性

Phospho-Histone H1 (T17) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when phosphorylated at T17.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

31kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

组织表达

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

细胞定位

Nucleus. Chromosome. According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.

信号通路

Protein_Acetylation

功能

function:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.,similarity:Belongs to the histone H1/H5 family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using Histone H1 （Phospho-Thr17） Antibody

Immunofluorescence analysis of HUVEC cells treated with serum 20% 30‘, using Histone H1 （Phospho-Thr17） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H1 （Phospho-Thr17） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with UV 15‘, using Histone H1 （Phospho-Thr17） Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of SKOV3 using p-Histone H1 （T17） antibody. Antibody was diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.