BD-PP0920-Net (phospho Ser357) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Net (phospho Ser357) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0920

产品名称

Net (phospho Ser357) Rabbit Polyclonal Antibody

别名

ELK3; NET; SAP2; ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2

类别

常规抗体

基因名称

ELK3

蛋白名称

ETS domain-containing protein Elk-3

推荐应用

反应种属

Human,Mouse,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2004

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2004

Human Swissprot No.

P41970

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P41970/entry

Mouse Gene ID

13713

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13713

Mouse Swissprot No.

P41971

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P41971

免疫原

The antiserum was produced against synthesized peptide derived from human Elk3 around the phosphorylation site of Ser357. AA range:323-372

特异性

Phospho-Net (S357) Polyclonal Antibody detects endogenous levels of Net protein only when phosphorylated at S357.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

38kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

组织表达

Brain,Epithelium,Placenta,

细胞定位

Nucleus.

功能

function:May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts with CTBP1.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using Elk3 （Phospho-Ser357） Antibody

Immunofluorescence analysis of HeLa cells, using Elk3 （Phospho-Ser357） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 （Phospho-Ser357） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15‘, using Elk3 （Phospho-Ser357） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of COLO cells using Phospho-Net （S357） Polyclonal Antibody