产品名称
CHOP (phospho Ser30) Rabbit Polyclonal Antibody
别名
DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153
蛋白名称
DNA damage-inducible transcript 3 protein
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1649
Human Swissprot No.
P35638
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P35638/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13198
Mouse Swissprot No.
P35639
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P35639
免疫原
The antiserum was produced against synthesized peptide derived from human CHOP around the phosphorylation site of Ser30. AA range:15-64
特异性
Phospho-CHOP (S30) Polyclonal Antibody detects endogenous levels of CHOP protein only when phosphorylated at S30.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],
组织表达
Muscle,Skeletal muscle,
细胞定位
Cytoplasm . Nucleus . Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation. .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;
功能
disease:A chromosomal aberration involving DDIT3 is found in a form of malignant myxoid liposarcoma [MIM:126337]. Translocation t(12;16)(q13;p11) with FUS.,function:Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
