产品名称
Bcr (phospho Tyr360) Rabbit Polyclonal Antibody
别名
BCR; BCR1; D22S11; Breakpoint cluster region protein; Renal carcinoma antigen NY-REN-26
蛋白名称
Breakpoint cluster region protein
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=613
Human Swissprot No.
P11274
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P11274/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=110279
Mouse Swissprot No.
Q6PAJ1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6PAJ1
免疫原
The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr360. AA range:331-380
特异性
Phospho-Bcr (Y360) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y360.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Brain,Epithelium,Platelet,Renal cell carcinoma,T-cell,
细胞定位
Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, axon . Cell junction, synapse .
信号通路
Pathways in cancer;Chronic myeloid leukemia;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,domain:The DH domain is involved in interaction with CCPG1.,domain:The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.,function:GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.,PTM:Autophosphorylated.,similarity:Contains 1 C2 domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 Rho-GAP domain.,subunit:Homotetramer. Interacts with PDZK1. May interact with CCPG1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
