BD-PP0878-Ets-1 (phospho Thr38) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Ets-1 (phospho Thr38) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0878

产品名称

Ets-1 (phospho Thr38) Rabbit Polyclonal Antibody

别名

ETS1; EWSR2; Protein C-ets-1; p54

类别

常规抗体

基因名称

ETS1

蛋白名称

Protein C-ets-1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2113

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2113

Human Swissprot No.

P14921

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P14921/entry

Mouse Gene ID

23871

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23871

Mouse Swissprot No.

P27577

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P27577

Rat Gene ID

24356

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24356

Rat Swissprot No.

P41156

Rat Swissprot Link

http://www.uniprot.org/uniprot/P41156

免疫原

The antiserum was produced against synthesized peptide derived from human ETS1 around the phosphorylation site of Thr38. AA range:11-60

特异性

Phospho-Ets-1 (T38) Polyclonal Antibody detects endogenous levels of Ets-1 protein only when phosphorylated at T38.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

54kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011],

组织表达

Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

细胞定位

Cytoplasm . Nucleus . Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27. .

信号通路

Dorso-ventral axis formation;Pathways in cancer;Renal cell carcinoma;

功能

disease:ETS is responsible for erythroblast and fibroblast transformation. The juxtaposition of the interferon and c-ETS-1 proto-oncogene may be involved in the pathogenesis of human monocytic leukemia.,function:Transcription factor.,PTM:Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.,PTM:Ubiquitinated; which induces proteasomal degradation.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,similarity:Contains 1 PNT (pointed) domain.,subunit:Interacts with MAF and MAFB (By similarity). Binds to DAXX. Interacts with UBE2I.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using ETS1 （Phospho-Thr38） Antibody

Immunofluorescence analysis of HeLa cells, using ETS1 （Phospho-Thr38） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ETS1 （Phospho-Thr38） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with Serum 20% 15‘, using ETS1 （Phospho-Thr38） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Ets-1 （T38） Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of VEC cells using Phospho-Ets-1 （T38） Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.