BD-PP0870-EF-2 (phospho Thr56) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EF-2 (phospho Thr56) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0870

产品名称

EF-2 (phospho Thr56) Rabbit Polyclonal Antibody

别名

EEF2; EF2; Elongation factor 2; EF-2

类别

常规抗体

基因名称

EEF2

蛋白名称

Elongation factor 2

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1938

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1938

Human Swissprot No.

P13639

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P13639/entry

Mouse Gene ID

13629

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13629

Mouse Swissprot No.

P58252

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P58252

Rat Gene ID

29565

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29565

Rat Swissprot No.

P05197

Rat Swissprot Link

http://www.uniprot.org/uniprot/P05197

免疫原

The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80

特异性

Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

100kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],

组织表达

Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph

细胞定位

Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .

信号通路

AMPK

功能

function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using eEF2 （Phospho-Thr56） Antibody

Immunofluorescence analysis of HUVEC cells, using eEF2 （Phospho-Thr56） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 （Phospho-Thr56） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30‘, using eEF2 （Phospho-Thr56） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of SKOV3 cells using Phospho-EF-2 （T56） Polyclonal Antibody diluted at 1：2000