BD-PP0869-Crk-L (phospho Tyr207) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Crk-L (phospho Tyr207) Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PP0869

产品名称

Crk-L (phospho Tyr207) Rabbit Polyclonal Antibody

别名

CRKL; Crk-like protein

类别

常规抗体

基因名称

CRKL

蛋白名称

Crk-like protein

推荐应用

反应种属

Human,Mouse,Rat,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1399

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1399

Human Swissprot No.

P46109

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P46109/entry

Mouse Gene ID

12929

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12929

Mouse Swissprot No.

P47941

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P47941

Rat Gene ID

100911000

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100911248

Rat Swissprot No.

Q5U2U2

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q5U2U2

免疫原

The antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222

特异性

Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

39kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],

组织表达

Skin,Spleen,

细胞定位

endosome,cytosol,cell-cell adherens junction,extracellular exosome,

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;Chemokine;Focal adhesion;Fc gamma R-mediated phagocytosis;Neurotrophin;Regulates Actin and Cytoskeleton;Insulin_Receptor;Pathways in cancer;Renal cell carcinoma;Chronic myeloid leukemia;

功能

function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using CrkL （Phospho-Tyr207） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CrkL （Phospho-Tyr207） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using CrkL （Phospho-Tyr207） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Crk-L （Y207） Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.