产品名称
POLK Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/51426
Human Swissprot No.
Q9UBT6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UBT6/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/27015
Mouse Swissprot No.
Q9QUG2
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9QUG2
免疫原
Synthesized peptide derived from human POLK AA range: 272-322
特异性
This antibody detects endogenous levels of POLK at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016],
组织表达
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
细胞定位
Nucleus . Detected throughout the nucleus and at replication foci (PubMed:12414988). Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716). .
功能
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Divalent metal cations. Prefers magnesium, but can also use manganese.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,similarity:Contains 2 Rad18-type zinc fingers.,subcellular location:Detected throughout the nucleus and at replication foci.,subunit:Binds REV1L (By similarity). Binds PCNA.,tissue specificity:Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
