产品名称
Cytokeratin 18 (phospho Ser33) Rabbit Polyclonal Antibody
别名
KRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
蛋白名称
Keratin type I cytoskeletal 18
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3875
Human Swissprot No.
P05783
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P05783/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16668
Mouse Swissprot No.
P05784
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P05784
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=294853
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5BJY9
免疫原
The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33. AA range:1-50
特异性
Phospho-Cytokeratin 18 (S33) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S33.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
细胞定位
Cytoplasm, perinuclear region. Nucleus, nucleolus.
信号通路
Pathogenic Escherichia coli infection;
功能
disease:Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.,induction:By IL-6.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.,PTM:Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.,tissue specificity:Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
