BD-PP0857-HSP90β (phospho Ser254) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HSP90β (phospho Ser254) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0857

产品名称

HSP90β (phospho Ser254) Rabbit Polyclonal Antibody

别名

HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

类别

常规抗体

基因名称

HSP90AB1

蛋白名称

Heat shock protein HSP 90-beta

推荐应用

反应种属

Human,Mouse,Rat,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3326

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3326

Human Swissprot No.

P08238

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P08238/entry

Mouse Gene ID

15516

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15516

Mouse Swissprot No.

P11499

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P11499

Rat Gene ID

301252

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=301252

Rat Swissprot No.

P34058

Rat Swissprot Link

http://www.uniprot.org/uniprot/P34058

免疫原

The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275

特异性

Phospho-HSP90β (S254) Polyclonal Antibody detects endogenous levels of HSP90β protein only when phosphorylated at S254.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

90kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],

组织表达

Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon

细胞定位

Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). .

信号通路

PI3K/Akt; Protein_Acetylation

功能

function:Molecular chaperone. Has ATPase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with TP53/p53 (By similarity). Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using HSP90B （Phospho-Ser254） Antibody

Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15‘, using HSP90B （Phospho-Ser254） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B （Phospho-Ser254） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30‘, using HSP90B （Phospho-Ser254） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-HSP90β （S254） Polyclonal Antibody

Western Blot analysis of HepG2 cells using Phospho-HSP90β （S254） Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.