BD-PP0853-14-3-3 ζ (phospho Ser58) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

14-3-3 ζ (phospho Ser58) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0853

产品名称

14-3-3 ζ (phospho Ser58) Rabbit Polyclonal Antibody

别名

YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

类别

常规抗体

基因名称

YWHAZ

蛋白名称

14-3-3 protein zeta/delta

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7534

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7534

Human Swissprot No.

P63104

Human Swissprot Link

http://www.uniprot.org/uniprot/P63104

Mouse Gene ID

22631

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22631

Mouse Swissprot No.

P63101

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P63101

Rat Gene ID

25578

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25578

Rat Swissprot No.

P63102

Rat Swissprot Link

http://www.uniprot.org/uniprot/P63102

免疫原

The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73

特异性

Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58.

稀释度

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

参考分子量

30KD

宿主

Rabbit

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using 14-3-3 zeta （Phospho-Ser58） Antibody

Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30, using 14-3-3 zeta （Phospho-Ser58） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta （Phospho-Ser58） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with UV 30‘, using 14-3-3 zeta （Phospho-Ser58） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-14-3-3 ζ （S58） Polyclonal Antibody diluted at 1：2000

Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ （S58） Polyclonal Antibody diluted at 1：2000