产品名称
UBF (phospho Ser484) Rabbit Polyclonal Antibody
别名
UBTF; UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1
蛋白名称
Nucleolar transcription factor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7343
Human Swissprot No.
P17480
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P17480/entry
Mouse Swissprot No.
P25976
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P25976
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25574
Rat Swissprot Link
http://www.uniprot.org/uniprot/P25977
免疫原
The antiserum was produced against synthesized peptide derived from human UBF around the phosphorylation site of Ser484. AA range:451-500
特异性
Phospho-UBF (S484) Polyclonal Antibody detects endogenous levels of UBF protein only when phosphorylated at S484.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011],
组织表达
Lymph,Placenta,Testis,
细胞定位
Nucleus, nucleolus .
功能
function:Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor SL1. It binds specifically to the upstream control element.,PTM:Phosphorylated and activated by PIK3CA.,similarity:Contains 6 HMG box DNA-binding domains.,subunit:Homodimer. Binds to IRS1 and PIK3CA.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
