产品名称
Mnk1 (phospho Thr385) Rabbit Polyclonal Antibody
别名
MKNK1; MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1
蛋白名称
MAP kinase-interacting serine/threonine-protein kinase 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8569
Human Swissprot No.
Q9BUB5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BUB5/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17346
Mouse Swissprot No.
O08605
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O08605
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=500526
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q4G050
免疫原
The antiserum was produced against synthesized peptide derived from human Mnk1 around the phosphorylation site of Thr385. AA range:351-400
特异性
Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
MAP kinase interacting serine/threonine kinase 1(MKNK1) Homo sapiens This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012],
细胞定位
[Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Insulin_Receptor;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.,function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
