产品名称
Tak1 (phospho Ser439) Rabbit Polyclonal Antibody
别名
MAP3K7; TAK1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1
蛋白名称
Mitogen-activated protein kinase kinase kinase 7
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6885
Human Swissprot No.
O43318
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43318/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26409
Mouse Swissprot No.
Q62073
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q62073
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100910771
Rat Swissprot Link
http://www.uniprot.org/uniprot/P0C8E4
免疫原
The antiserum was produced against synthesized peptide derived from human MAP3K7 around the phosphorylation site of Ser439. AA range:411-460
特异性
Phospho-Tak1 (S439) Polyclonal Antibody detects endogenous levels of Tak1 protein only when phosphorylated at S439.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],
组织表达
Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
细胞定位
Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Although the majority of MAP3K7/TAK1 is found in the cytosol, when complexed with TAB1/MAP3K7IP1 and TAB2/MAP3K7IP2, it is also localized at the cell membrane.
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;WNT;WNT-T CELLAdherens_Junction;Toll_Like;NOD-like receptor;RIG-I-like receptor;T_Cell_Receptor;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF-kappa-B activation and the p38 MAPK pathway.,PTM:Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2. Interacts with PPM1L. Interaction with PP2A and PPP6C leads to its' repressed activity.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
