产品名称
MEK Kinase-1 (phospho Thr1402) Rabbit Polyclonal Antibody
别名
MAP3K1; MAPKKK1; MEKK; MEKK1; Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1
蛋白名称
Mitogen-activated protein kinase kinase kinase 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4214
Human Swissprot No.
Q13233
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13233/entry
Mouse Swissprot No.
P53349
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P53349
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116667
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q62925
免疫原
The antiserum was produced against synthesized peptide derived from human MAP3K1 around the phosphorylation site of Thr1402. AA range:1368-1417
特异性
Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody detects endogenous levels of MEK Kinase-1 protein only when phosphorylated at T1402.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012],
信号通路
SAPK_JNK; Regulation of Actin Dynamics; Cell Growth; Stem cell pathway; MAPK_ERK_Growth;MAPK_G_Protein; B Cell Receptor
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by autophosphorylation on Thr-1400 and Thr-1412 following oligomerization.,function:Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SWIM-type zinc finger.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes through its N-terminus. Oligomerizes after binding MAP4K2 or TRAF2. Interacts with AXIN1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
