产品名称
Casein Kinase Iα (phospho Tyr294) Rabbit Polyclonal Antibody
别名
CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1; CSNK1A1L; Casein kinase I isoform alpha-like; CKI-alpha-like; CK1
蛋白名称
Casein kinase I isoform alpha
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
1452/122011
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1452
Human Swissprot No.
P48729/Q8N752
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P48729/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=113927
Rat Swissprot Link
http://www.uniprot.org/uniprot/P97633
免疫原
The antiserum was produced against synthesized peptide derived from human CK-1 alpha around the phosphorylation site of Tyr294. AA range:260-309
特异性
Phospho-Casein Kinase Iα (Y294) Polyclonal Antibody detects endogenous levels of Casein Kinase Iα protein only when phosphorylated at Y294.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
CSNK1A1 (Casein Kinase 1 Alpha 1) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Infectious disease. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CSNK1G2. asein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at Ser-45. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed: 11955436, PubMed: 1409656, PubMed: 18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed: 23902688). asein kinase I (CK1) is a monomeric serine-threonine protein kinase with 7 isoforms: alpha, beta, gamma1, gamma2, gamma3, delta and epsilon. CK1 is involved in many cellular processes including DNA repair, cell division, nuclear localization and membrane transport. Isoforms are also integral to development.
组织表达
Brain,Epithelium,Liver,Lung,Placenta,Skin,
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . Nucleus speckle . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, spindle . Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688). .
信号通路
WNT;WNT-T CELLHedgehog;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
