产品名称
SRF (phospho Ser77) Rabbit Polyclonal Antibody
别名
SRF; Serum response factor; SRF
蛋白名称
Serum response factor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6722
Human Swissprot No.
P11831
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P11831/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20807
Mouse Swissprot No.
Q9JM73
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JM73
免疫原
The antiserum was produced against synthesized peptide derived from human SRF around the phosphorylation site of Ser77. AA range:41-90
特异性
Phospho-SRF (S77) Polyclonal Antibody detects endogenous levels of SRF protein only when phosphorylated at S77.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014],
组织表达
Brain,Epithelium,Lung,Lymph,
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;
功能
function:SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentation and maturation.,PTM:Phosphorylated by PRKDC.,similarity:Contains 1 MADS-box domain.,subunit:Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1 (By similarity). Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
