产品名称
Pdcd-4 (phospho Ser457) Rabbit Polyclonal Antibody
别名
PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
蛋白名称
Programmed cell death protein 4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27250
Human Swissprot No.
Q53EL6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q53EL6/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18569
Mouse Swissprot No.
Q61823
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61823
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64031
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9JID1
免疫原
The antiserum was produced against synthesized peptide derived from human PDCD4 around the phosphorylation site of Ser457. AA range:420-469
特异性
Phospho-Pdcd-4 (S457) Polyclonal Antibody detects endogenous levels of Pdcd-4 protein only when phosphorylated at S457.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],
组织表达
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
细胞定位
Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). .
功能
caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Loss of expression correlated with tumor progression of lung and colon carcinoma.,domain:Binds EIF4A1 via the MA3 domains.,function:Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Inhibits the helicase activity of EIF4A and cap-dependent translation. Binds RNA.,induction:IL2 stimulation inhibits expression, while IL12 increases expression.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the PDCD4 family.,similarity:Contains 2 MI domains.,subcellular location:Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions. Exported from the nucleus in the absence of serum.,subunit:Interacts with EIF4A1 and EIF4A2.,tissue specificity:Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
