产品名称
Rad52 (phospho Tyr104) Rabbit Polyclonal Antibody
别名
RAD52; DNA repair protein RAD52 homolog
蛋白名称
DNA repair protein RAD52 homolog
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5893
Human Swissprot No.
P43351
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P43351/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19365
Mouse Swissprot No.
P43352
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P43352
免疫原
The antiserum was produced against synthesized peptide derived from human RAD52 around the phosphorylation site of Tyr104. AA range:70-119
特异性
Phospho-Rad52 (Y104) Polyclonal Antibody detects endogenous levels of Rad52 protein only when phosphorylated at Y104.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014],
组织表达
Amygdala,Brain,Embryonal rhabdomyosarcoma,Hippocampus,PCR rescued c
信号通路
Homologous recombination;
功能
function:Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RAD52 family.,subunit:Forms a undecameric ring.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
