产品名称
GRK 1 (phospho Ser21) Rabbit Polyclonal Antibody
别名
GRK1; RHOK; Rhodopsin kinase; RK; G protein-coupled receptor kinase 1
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6011
Human Swissprot No.
Q15835
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15835/entry
Mouse Swissprot No.
Q9WVL4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9WVL4
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81760
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63651
免疫原
The antiserum was produced against synthesized peptide derived from human GRK1 around the phosphorylation site of Ser21. AA range:6-55
特异性
Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008],
组织表达
Retinal-specific. Expressed in rods and cones cells.
细胞定位
Membrane ; Lipid-anchor . Cell projection, cilium, photoreceptor outer segment . Subcellular location is not affected by light or dark conditions. .
信号通路
Chemokine;Endocytosis;
功能
catalytic activity:ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.,disease:Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type (CSNBO) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation.,function:Phosphorylates rhodopsin thereby initiating its deactivation.,online information:Retina International's Scientific Newsletter,PTM:Autophosphorylated.,PTM:Farnesylation is required for full activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,tissue specificity:Retina and pineal gland.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
