BD-PP0739-FAK (phospho Tyr397) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FAK (phospho Tyr397) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0739

产品名称

FAK (phospho Tyr397) Rabbit Polyclonal Antibody

别名

PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

类别

常规抗体

基因名称

PTK2

蛋白名称

Focal adhesion kinase 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5747

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5747

Human Swissprot No.

Q05397

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q05397/entry

Mouse Gene ID

14083

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14083

Mouse Swissprot No.

P34152

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P34152

Rat Gene ID

25614

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25614

Rat Swissprot No.

O35346

Rat Swissprot Link

http://www.uniprot.org/uniprot/O35346

免疫原

The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397. AA range:363-412

特异性

Phospho-FAK (Y397) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y397.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

预测分子量

119kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],

组织表达

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787).

细胞定位

Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.

信号通路

ErbB_HER;Chemokine;Axon guidance;VEGF;Focal adhesion;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;Pathways in cancer;Small cell lung cancer;

功能

catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.,domain:The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.,function:Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.,PTM:Phosphorylated on 6 tyrosine residues upon activation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.,similarity:Contains 1 FERM domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Constituent of focal adhesions.,subunit:Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB (By similarity). Interacts with TGFB1I1.,tissue specificity:Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human brain, using FAK （Phospho-Tyr397） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with Ca2+ 40nM 30‘, using FAK （Phospho-Tyr397） Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,FAK （phospho Tyr397） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,FAK （phospho Tyr397） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,FAK （phospho Tyr397） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Phospho-FAK （Y397） Polyclonal Antibody diluted at 1：1000

Western Blot analysis of 293T cells using Phospho-FAK （Y397） Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.