产品名称
TF (phospho Ser290) Rabbit Polyclonal Antibody
别名
F3; Tissue factor; TF; Coagulation factor III; Thromboplastin; CD antigen CD142
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2152
Human Swissprot No.
P13726
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P13726/entry
Mouse Swissprot No.
P20352
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P20352
免疫原
The antiserum was produced against synthesized peptide derived from human Coagulation Factor III around the phosphorylation site of Ser290. AA range:246-295
特异性
Phospho-TF (S290) Polyclonal Antibody detects endogenous levels of TF protein only when phosphorylated at S290.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
组织表达
Lung, placenta and pancreas.
细胞定位
[Isoform 1]: Membrane ; Single-pass type I membrane protein .; [Isoform 2]: Secreted .
信号通路
Complement and coagulation cascades;
功能
function:Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.,induction:TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response.,online information:The Singapore human mutation and polymorphism database,online information:Tissue factor entry,similarity:Belongs to the tissue factor family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
